Sentence examples for using an imaging flow from inspiring English sources
In order to understand the differences between toxin-treated and control cells in G2/M, we determined several cellular characteristics (circumference, area, and others) of individual cells using an imaging flow cytometer.
To demonstrate our concept Qtracker® 705 nm CdTe/ZnS (Invitrogen) quantum dots are loaded into the endosomal compartments of U2-OS cells and subsequently the fluorescence intensity of each cell is measured using an imaging flow cytometer; i.e. the measured parameter is total fluorescence intensity and the inherited cellular material are the QD loaded endosomes.
Using an imaging spectrometer on the MRO, NASA identified hydrated minerals with patterns unique to flowing water.
We have developed a high throughput, statistically robust technique that quantitates autophagy in primary human leukocytes using the Image stream, an imaging flow cytometer.
We measured titers of infectious virus by staining the KSHV-exposed HeLa cells for LANA and analyzing cells using multispectral imaging flow cytometry (MIFC) to determine the number of punctate LANA dots per cell.
To determine the intracellular trafficking pattern of the internalized antibody-CAIX complexes, anti-CAIX scFvFc G36 and G119 were selected for co-localization studies of CypHer5E-labeled anti-CAIX antibodies to endosomes (stained with FITC anti-EEA1) or lysosomes (stained with PE anti-LAMP1) using quantitative imaging flow cytometry (Figure 6).
The morphology of stained nuclei was evaluated on the one hand by visual consideration, and on the other hand by an objective quantification of the area and bright detail intensity of the nuclei stain using multispectral imaging flow cytometry (MIFC).
Using the imaging flow cytometer (ImageStream) to count LC3 puncta in CD4+ and CD8+ T cells (Phadwal et al., 2012), we demonstrated that functional autophagy was significantly diminished in Atg7 −/− CD8+ T cells.
Single-cell images were acquired using imaging flow cytometry, and nuclear translocation quantified in approximately 1,000 images captured per sample using IDEAS image analysis software (Amnis).
We have previously demonstrated that measuring the shape of the nucleus from cells stained with a nuclear marker using imaging flow cytometry drastically improves the classification of mitotic phases9.
The rich information captured using imaging flow cytometry makes it an ideal candidate for the use of high content approaches to identify complex cell phenotypes such as the cell cycle phase of an individual cell.
