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Totally, 10 random areas were quantified using an imaging analyzing system (CUE-2, Olympus Opticals, Hamburg, Germany), and the numbers were normalized to retinal capillary area.
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Signal intensities were analyzed using an imaging analyzer LAS-3000minii; Fujifilm) and Multi Gauge software (version 3.0; Fujifilm).
The ECL signals were recorded using an imaging system (LAS4000, USA) and analyzed using Quantity One (Bio-Rad, USA).
Blots were analyzed using an Odyssey imaging system.
The gel was neutralized with TAE for 1 h, incubated with ethidium bromide solution, and analyzed using an LAS-3000 imaging system.
Changes in fluorescence were analyzed using an Openlab imaging system and the average pixel value for each cell was measured at excitation wavelengths of 340 and 380 nm as previously described [Ducreux et al., 2004].
Quantification of the intensity of the bands in the autoradiograms was performed using a Kodak imaging system and analyzed by SCION IMAGE.
These arrays have been analyzed using a hyperspectral imaging system capable of measuring biological samples [ 5].
Immediately after this, the chemoluminescent agent was removed and the PVDF membrane was analyzed using a digital imaging system (G Box, Syngene, MD, USA).
TLC plates were analyzed using a Bioscan imaging detector (Bioscan, Washington, DC, USA) and the fractional conversion of steroids was calculated.
The gel was stained with ethidium bromide and analyzed using a UV-imaging system (Fluorochem 5330).
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