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Immunostained slides were scanned with a digital virtual microscope (dotSlide, Olympus, Hamburg, Germany), and a portion (area of 0.44 mm of tumour centre) of the virtual images was captured using an image viewer programme (OlyVIA, downloaded from http://www.olysia.co.kr/olyvia2992.zip) per case.
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Images were analysed using OlyVia Image Viewer 2.6 (Olympus).
Individual 3D were hand drawn on T1-weighted templates for each slice using FMRIB Software Library image viewer (FSLView).
Image processing was done using the Axiovision Image viewer (Carl Zeiss, Germany).
The OPT images can be viewed in virtual section mode using the IIP3D image viewer (Husz et al., 2012).
These images were transmitted to a personal computer in the digital imaging and communications in medicine (DICOM) format and reconstructed into multiplanar reconstruction images using a DICOM viewer (ExaVision SX version 1.13; Ziosoft Inc., Tokyo, Japan).
Images of individual sections of each set retrieved can be inspected using image viewers that are integrated into the database.
Using a slide viewer.
A skilled operator examined each of the confocal image stacks interactively, first using a 2D slice viewer and then a 3D volume rendering.
In order to analyse the movements of chitinase molecules along crystalline chitin fibrils, kymographs were constructed using a laboratory-developed AFM image viewer based on Igor Pro (Ver. 6.22, WaveMetrics Inc.).
An internet website was then constructed from this database by using a WebSlide-Viewer Java applet provided by the manufacturer to view the microarray images and to permit an image-zooming functionality.
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