Collagen content of biopsies was estimated by image analysis of the Masson's trichrome stain using an image software system (AnalySIS™, Soft Imaging System, Munster, Germany).
The number of nuclei seen within one frame for one section stained with HE system was counted using an image analysis system.
Tissue sections stained with haematoxylin and eosin (HE) and Masson's trichrome underwent morphometric studies using an image analysis system (Leica Q500 iW; Leica Imaging Systems, Cambridge, UK).
The areas of injured (white) and uninjured (red) were measured using an image analysis system (Adobe Photoshop 8.0, Adobe Systems Incorporated, San Jose, CA) for each slice.
The size and shape factor distributions were measured using an image analysis system on SEM pictures.
Subsequently, the area of each wound was measured from video by using an image analysis system.
They evaluated the fabric appearance using an image processing system.
Ring-widths were measured from the scanned images using an image analysis system (WinDENDRO, Regent Instruments, Québec, Canada).
Histological measurements were performed on sections using an image analyzing system (Scion Image, NIH, Bethesda, MD, USA).
After substraction of background for non-specific staining, densitometric analysis of autoradiographs was performed using an image analysis system (Densirag V2.0, Explora Nova, La Rochelle, France).
