A filament was tracked from the center of mass, and the speed was calculated from ten frames at an acquisition rate of five or one frame (s) per second, depending on the fiber type, using an image analysis package (Image-pro Plus Version 6.0; Media Cybernetics, USA) (Hook & Larsson, 2000).
To compensate the variation, we measured the intensity of each fiber pixel by using an image processing function of MATLAB to obtain weight functions that are applied to raw images to produce thermal distribution in measurements of thermal images of samples.
Based on SEM images, the fiber diameters were analyzed using an image visualization software ImageJ 1.501 developed by Wayne Rasband at the National Institutes of Health, USA.
Fiber length distributions of composites were also determined by using an image analyzing program.
The number of each fiber type was assessed using an image-processing program (ImageJ 1.40, National Institutes of Health, Bethesda, MD).
The morphologies of the electrospun fibers were observed using a scanning electron microscope (SEM) JSM-6390LVV, JEOLtd.d., Akishima, Tokyo, Japan), and the fiber diameter was measured from the SEM image using an image-analyzer software (Image J 1.42).
However a recent approach used an image-preserving fiber bundle to relay the laparoscope output image onto a sensitive electron-multiplying CCD [ 38], rather than mounting the detector directly on the laparoscope.
Use an image capture program.
In addition to the bioluminescence image, a transmission image (or images) is acquired using an external fiber optic source (or sources) of known optical power.
An endoscope system was designed and developed, using image fiber as the speckle pattern image conduit, to work in the out-of-plane speckle interferometric configuration and experiments were carried out using a curved phantom tissue specimen as the test target.
