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Using an identical preincubation protocol of Cu-Phen (10 min preincubation and 5 min wash at −80 mV), a step hyperpolarization to −140 mV did not produce any inward K+ current (Figure 4E, lower panel, black trace before the arrow).
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Thus, information for the referents and pregnant women was obtained using an identical questionnaire and virtually identical routines.
Moreover, each bank used an identical interface in its communications with MAC.
Each agent used an identical set of axiomatic operating principles.
Like for mutant I227C, using a preincubation protocol with Cu-Phe (10 min preincubation and 5 min wash at −80 mV), we found that opening the R228C channels by a step depolarization to +30 mV produced a slowly developing time- and voltage-dependent K+ current (red trace before the arrow) without any instantaneous component.
Non-specific binding was reduced using a 30 minute preincubation in 0.3% bovine serum albumin (BSA) in 0.1 M tris-buffered saline (TBS, pH = 8) with 0.05% Tween (TBS-T).
For the incubations using an extended duration of preincubation, serum-containing medium (final FCS concentration 10%) was added after 3 h.
From there, they can sell almost identical products using almost an identical marketing strategy as the one used by the original brand.
From there, they can sell almost identical products using almost an identical marketing strategy to the one used by the original brand.
Using a modified, two-step preincubation protocol with Salmonella, we have tested this hypothesis.
Texas uses a virtually identical formula.
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