Sentence examples for using an extension time from inspiring English sources

An 18 kb PCR product was obtained using an extension time of 24 minutes for each cycle but not using 20 minutes extension time (Additional file 3).

Most primer pairs (full list in Additional file 1) amplified products in the range of 500 1000 bp and, thus, we used an extension time of approximately 0 30.

All PCR reactions were performed with Biotools DNA polymerase (Biotools, B&M Labs, Spain); the PCR programme consisted of initial denaturation for 5 min at 94°C followed by 30 cycles of 30 sec at 94°C, annealing for 30 sec at a temperature dependent on the Tm of the primers used, and an extension time at 72°C dependent on the size of amplicon.

An annealing temperature of 50°C was used for all oligonucleotides with an extension time of 30 seconds.

The 3,272-bp 3,272-bp was amplifragmenth 30 cycles by using an annealing temperature of 50°C and an extension time of 3 min, 20 s.

All primer combinations used an annealing temperature of 50°C and an extension time of two minutes.

Annealing temperature used was 3° below primer Tm with an extension time of 1 min 30 sec.

35 cycles of PCR in a non-linear, saturating range of amplification were carried out with an annealing temperature of 60°C and an extension time of 30 seconds using standard cycling conditions.

Be very careful in any situation where you use an extension cord for extended periods of time.

It's so bad that I use an extension to turn them off most of the time.

Do not use an extension cord.