Sentence examples for using an expression vector from inspiring English sources

To test the activity of LesBMUT1/LesBMUT2 in vitro, we expressed LesB and LesBMUT1/LesBMUT2 in E. coli using an expression vector to obtain high quantities of heterologous protein.

The clotting factor VIII (FVIII) and cystic fibrosis transmembrane conductance regulator (CFTR) cDNAs have dramatically reduced levels of expression compared to clotting factor IX (FIX) and other cDNAs (100 and 1,000-fold 1,000-foldpectivelowerwhen prespectivelyells by using an expression vector.

However, the mature CssB protein was also fused to the 26 kDa glutathione-S-transferase (sj26GST) from Schistosoma japonicum using an expression vector constructed in this laboratory.

We overexpressed miR-127 in human Hela and mouse Hepa-1 cells using an expression vector of miR-127 and the level of Rtl1 was assessed using a strand specific q-PCR analysis.

A 351 base pair DNA fragment was amplified by PCR using an expression vector for the MBP 14 kDa isoform [14] with the following oligonucleotides 5'-ATGGCATCACAGAAGAGACC-3' and 5'- TCTTCCTCCCCAGCTAAATC-3'.

In vitro over-expression of the human, rat, or dog miR-433/127 loci in cells, using an expression vector containing miR-433/127 and their promoter regions, markedly induced a differential expression of both primary and mature miR-433 and miR-127, indicating that miR-433 and miR-127 were possessed from their independent promoters.

We distinguished between the expression of endogeneous and exogenous pch-2 by using an expression vector-specific primer and a pch-2-specific primer.

Tsujibo et al. (2001) used an expression vector pET-20b to overexpress a β-Xylosidase gene (bxlA) with no typical −10 and −35 promoter boxes from Streptomyces thermoviolaceus in E. coli BL21 (DE3) pLysE.

Because none of the breast cancer cell lines presented aberrant TCF/LEF transcription, we used an expression vector encoding a β-catenin mutant to boost Wnt signalling.

To corroborate that our finding of increased expression of autophagic markers in ER α cells reflect an enhanced autophagosome biogenesis rather than a decreased autophagosome clearance, we used an expression vector (ptfLC3) encoding LC3 fused to red fluorescent protein (RFP) and green fluorescent protein (GFP) in tandem (GFP-RFP-LC3).

Functional validations using either an expression vector encoding for S100A4 or siRNA against its RNA show a role for S100A4 in MTX resistance.