Sentence examples for using an expression system from inspiring English sources
Proteins were expressed in Escherichia coli using an expression system comprising the autoprotease Npro from Pestivirus, or its engineered mutant called EDDIE, with C-terminally linked target proteins.
To develop the Cap protein as coating antigen of an ELISA system, the above mentioned limitations associated with using an E. coli expression system need to be overcome; these include making sure that the full-length of the Cap protein is expressed in E. coli and using an expression system in which the majority of Cap protein is produced in a soluble form rather than as inclusion bodies.
We prepared recombinant samples of AaegOBP1 by using an expression system that allows proper formation of disulfide bridges and generates functional OBPs, which are indistinguishable from native OBPs.
Initial experiments were undertaken using an expression system in which the CRD was secreted from bacteria under the direction of the ompA signal sequence.
It should be noted that the VHH fragment should preferably be constitutively produced using an expression system devoid of antibiotic selection marker.
The authors could lower expression levels by using an expression system independent of the highly active Gal4/UAS system, by reducing the temperature (the Gal4 system is less active at 18C), or by co-expressing Gal80ts.
It is thus desirable to use an expression system without this sequence limitation.
To identify functional NLS motifs, we used an expression system that produces an N-terminal test peptide fused to the enhanced green fluorescence protein (EGFP) to determine nuclear targeting.
To provide support for these linkages on LRRK2, we used an expression system with ubiquitin mutants that allow conjugation through only one lysine residue, Lys or Lys, whereas all other lysines are mutated to arginine.
A chinese hamster ovary cell line often used as an expression system for studying cell signalling and recombinant proteins.
A hypotriploid human cell line derived from embryonic kidney epithelial cells; commonly used as an expression system to study signalling and recombinant proteins.
