AFP can also be used as an expression reporter assay; the gene of interest is either transcriptionally or translationally fused to AFP so that the expression level of AFP correlating either to the level of transcription or translation can be quantitatively measured by flow cytometry.
We catalogued the genes showing differential expression in the mutant skin and found some of them to be tightly regulated by HR, which we confirmed using a reporter expression system.
To facilitate reporter gene expression analysis during metamorphosis, and to increase the spatial and temporal characterization of the reporter expression pattern, we analyzed a subset using a fluorescent reporter expression assay.
As TLR3 activates and programs interferon and chemokine expression through the coordinated activation of IRF3 and NFκB [ 28, 29], we next examined the effect of I329L on the activation of CCL5 expression using an appropriate reporter plasmid.
The efficiency and fidelity of the 12 nitroTyr-RS variants in pDule were evaluated using a similar fluorescent expression reporter, but now the truncated sfGFP gene was encoded on a standard protein expression plasmid.
The potential role of either or both is supported by the heterologous experiments using an RNA reporter system, in which expression of either constitutively active Rac2 or Rac1 was sufficient to stabilize the otherwise destabilized, uPAR class II ARE-bearing β-globin transcript.
To identify the transcriptional factors that directly regulate AREG expression, we examined the transcriptional region controlling AREG expression using a luciferase reporter assay.
The ability to analyze gene expression using a standard reporter is an important and critically needed tool to study gene regulation and design genetic screens for C. difficile and other anaerobic clostridia.
These were examined for ability to alter expression using a luciferase reporter assay, and two regulatory SNPs, showing genotype differences, rs327 and rs3289, were identified.
Using a fluorescent reporter for Yan expression, we observed a biphasic distribution of Yan in multipotent cells, with a rapid inductive phase and slow decay phase.
