Sentence examples for using an expression cut-off from inspiring English sources

Using an expression cut-off of four TPM to focus on strong initiation events, 13,970 TCs were retained, of which 92% overlapped an annotated RefSeq gene model.

Expression patterns were identified by stringent data analysis using anticorrelation of the dye reversal ratio profiles and a 2-fold expression cut-off.

ER + status for the TCGA cohort was determined using expression cut-offs (250 RSEM normalised transcript counts) for the ESR1 gene.

Transcripts were identified with significantly altered expression using an FDR cut-off of 25%.

Significant changes in gene expression were determined using a 1.5-fold cut-off in expression change and a FDR of <25%.

Patients were grouped according to high/low tTP gene expression using a median value cut-off (0.20, IQR 0.080 0.51).

Using an expression ratio of over two-fold as cut-off, 22 of the 29 cDNA clones, and 99 of1199 cDNA clones examined by reverse Northern screening were identified in the benign and malignant subtracted libraries, respectively (Table 1-wrap>).

Since DNA damage responses often show small changes on the transcriptional level [ 24], we analysed the gene expression signatures using a fold-change cut-off criterion ≥1.8.

To offset some of the heterogeneity that arises from the inclusion of ER/PR negative cases in a consecutive series of patients, we next used the same expression cut-offs and looked within the ER-positive subgroup.

Gene expression values were normalized to the RB-proficient p53DD, untreated cells (GFP Naïve), and significant changes in gene expression were determined using a 1.5-fold cut-off and a FDR of <25%.

Using a >twofold-change cut-off for gene expression changes, 487 probe sets appeared up-regulated in adenomas versus normal wild-type gland, and 400 were down-regulated (Supplementary Fig. 1 and Dataset 1).