Sentence examples for using an expression construct from inspiring English sources

To verify that the YopK-RACK1 interaction observed in yeast also occurred in HeLa cells (our established eukaryotic cell model system), we performed pull down assays using an expression construct (YopK FLAG fusion) and HeLa cell lysates.

To investigate the potential post-transcriptional role and target transcripts of WT1 +KTS) in podocytes, we developed a modified RIP-CHIP approach using an expression construct originally developed for a tandem affinity purification (TAP) protocol [27].

By using an expression construct that encoded the entire Fascin-1-coding sequence, but lacked the 3′-UTR, mRNA that was resistant to miRNA-mediated suppression was produced.

Several assays were carried out using an expression construct encoding the BRCA1 TAD to ascertain any effects of R1699Q, A1708V, and G1738R on the capacity of the region to activate a co-transfected reporter construct in two independent mammalian cell lines.

Transgenic mice expressing the rat GlyR β gene thoughout the central nervous system were generated using an expression construct described earlier (Hartenstein et al. 1996), in which the rat β cDNA was joined to the neuron-specific enolase (NSE) promoter from rat and flanked by the SV40 small T antigen polyadenylation signal.

However, the mature CssB protein was also fused to the 26 kDa glutathione-S-transferase (sj26GST) from Schistosoma japonicum using an expression vector constructed in this laboratory.

To determine the subcellular localisation of Bcl-G, we first used 293T cells transiently transfected with an expression construct encoding full-length mouse Bcl-G.

This was tested further using an Uba80 expression construct created by placing Uba80 under the control of a tetracycline (tet -repressible promotet -repressible

Targeting ERCC1 expression, using an antisense expression construct, made ovarian cancer more sensitive to cisplatin than controls in cell line and xenograft models [ 188].

Notably, we were able to demonstrate robust pro-survival effects of KLK6 toward Jurkat T cells when cells were either treated with recombinant KLK6 or when KLK6 over expression was enforced using a KLK6 expression construct under control of a CMV promoter.

Rescue cells were generated using a p300 expression construct under the control of an EF-promoter (human elongation factor 1 α promoter).