Sentence examples for using an expression cloning from inspiring English sources

Using an expression cloning strategy we have identified a novel membrane bound aspartic protease, BACE1, as β-secretase.

It was later identified functionally as a molecule involved in B-cell lymphopoiesis using an expression cloning strategy.

The Fukuda group used an expression cloning strategy enriching for plasmids containing cDNA inserts that substantially increased poly-N-acetyllactosamine, as judged by antibody binding, on the surfaces of Namalwa KJM-1 cells (Sasaki et al., 1997).

Using the serological expression cloning approach we also identified bacterial antigens that are involved in these immune reactions.

We have now identified one of the major autoantibody specificities using expression cloning.

We have successfully used functional expression cloning [ 1] to identify novel genes playing controlling roles in the apoptosis process.

21, 22 Previously, we used expression cloning to isolate a human A4GNT cDNA encoding α1,4- N-acetylglucosaminyltransferase (α4GnT), the enzyme responsible for αGlcNAc biosynthesis.

As conventional Western blot and immunoprecipitation techniques had proven unsuccesful to give information on the identity of the antigen of this Mab, we used an eukaryotic expression cloning procedure.

The ABACUS optimization process was accelerated using a cloning, expression and purification platform that is readily applicable to the engineering of FRET sensors for other small molecules or molecular events (Table 1; Figure 1).

Using functional expression cloning and T-cell receptor (TCR) transgenic mice, we have identified cyclooxygenase 2/prostaglandin-endoperoxide synthase 2 (COX-2) as resistance factor against the cytotoxicity induced by activated, antigen-specific T cells.

According to our previous works, the systematic screening of P. furiosus genome using the expression cloning method has determined several DNA/RNA-binding proteins such as Thy-1 and FAU-1.