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Daily nutrient and energy intake was estimated from the food frequency questionnaire and the alcohol questionnaire using an expanded nutrient database (HHHQ-DIETSYS analysis software, version 3.0; National Cancer Institute, Bethesda, MD).
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DNA fragments were amplified using an Expand High Fidelity PCR Amplification System (Roche).
Nutrient intakes were computed using an in-house nutrient calculator in Microsoft Office Excel 2003 (Microsoft Corporation, Redmond, WA).
using a hydroponic nutrient film technique along with elevated CO2 (1000 or 1200 μmol mol−1).
Hydroponics basically involves growing plants without soil, instead using a reservoir of nutrient solutions in water.
Nano-MgO can be used as an expanding additive.
Vary the types of vegetables for interest and an expanded amount of nutrients.
You can also use an egg for nutrients.
Nutrient intake was estimated using an established food frequency questionnaire [19].
Fresh Intake's engineer, Chia-Yu Yen, also acknowledged the trade-off, and said, "Hydroponics uses few nutrients, but if we plant crops in the earth it uses a lot of nutrients.
Using an old-fashioned microbiological technique, he spread out some attack spores on a bed of nutrient and let each form its own colony.
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