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Using an expanded gold standard (defined as a true positive or a true negative where a specimen was positive or negative by at least two of the study assays, respectively), PCR had a clinical sensitivity and specificity of 46/46 (100%) and 15/126 (91.3%), respectively.
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C. trachomatis detection results of 553 cases with culture, LCR and enzymatic method used an expanded gold standard as the reference standard were showed in Table 4.
Furthermore, 553 urogenital tract specimens were used for clinical assays via cell culture method and ligase chain reaction method (LCR), adopting an expanded gold standard.
DNA fragments were amplified using an Expand High Fidelity PCR Amplification System (Roche).
Specifically, potentiostatic deposition of dendritic gold structures using a gold chloride solution, previously described by Lai and co-workers, yields gold electrode surfaces with significantly larger surface area.
Signals were detected using a colloid gold particle conjugated to anti-mouse IgG (10 nm diameter of conjugated gold; BB International).
Gold ore cannot be mined using a gold pickaxe.
Nano-MgO can be used as an expanding additive.
We have used a thick gold layer as the reference.
Use a brown, gold, or a slightly reddish-orange eyeshadow.
Use a light gold for the inner 3rd.
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