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It is noteworthy that polarized substratum-attached neutrophils produce bursts of O2− production (determined using an exogenous fluorescent probe for ROS) that correlated with waves of NAD(P H [determined by monitoring the autofluorescence of NAD(P H] that traveled from the rear to the front of crawling cells (Kindzelskii and Petty 2002).
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Based on visual observation of the laser-manipulated embryos, optimal laser parameters for transient pore formation and delivery of an exogenous fluorescent probe were defined.
Having established that a fluorescent ligand could be locally administered to NMJs and provide sufficient brightness and contrast to allow motor endplates to be visualized readily by CEM, we next asked whether high-contrast labeling of motor nerve terminals could be obtained using other exogenous fluorescent dyes.
Using the exogenous fluorescent phospholipid Rh-DOPE, Lopez et al. (2010) observed both fluorescent and non-fluorescent areas at the surface of bovine milk fat globules and interpreted this heterogeneity as the phase separation of polar lipids in the outer bilayer of the MFGM (Fig. 1h n).
Researchers were able to visualize precancerous tissues, tumors and metastasis in real-time and with high contrast by using exogenous fluorescent markers against the targeted structures.
The other modality, i.e. optical breast imaging with a contrast agent, uses exogenous fluorescent probes that target molecules specific for breast cancer.
In optical breast imaging, two techniques can be distinguished, i.e. optical imaging without contrast agent, which only makes use of intrinsic tissue contrast, and optical imaging with a contrast agent, which uses exogenous fluorescent probes.
Fluorescent images were captured using a BIOREVO fluorescence microscope.
Fluorescent images were obtained using an Olympus fluorescent microscope.
Fluorescent images were acquired using an EVOS inverted fluorescent digital microscope (Advanced Microscopy Group) with a ×10 objective.
Fluorescent cells were visualised using an Olympus IX81S8F fluorescent microscope at 40x magnification.
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