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In simple cases the amplitude and phase portions can be made separately, the phase filter being manufactured by using an evaporated layer of transparent material, such as magnesium fluoride.
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In a typical synthesis procedure, we used a gold layer evaporated on one side of the membrane as the working electrode, a platinum plate as the counter electrode and a saturated calomel electrode as the reference.
A 150-nm-thick Al gate metal layer was then evaporated using an electron beam evaporator.
The extracts were then partitioned with 100 ml of ethyl acetate and 100 ml water, and the ethyl acetate layer was evaporated using a rotary evaporator to give an ethyl acetate soluble.
Finally, the combined chloroform layers were evaporated using a rotary evaporator under reduced pressure to yield the chloroform extract of C. taii (CFCT, 210 g).
The filtrate was evaporated using a rotary evaporator at < 40°C to ca. 150 ml until all the methanol evaporated.
The organic layers were combined and the solvent was evaporated using a rotary evaporator.
The solvent was evaporated using a rotary evaporator.
Then, the liquid was evaporated completely using a rotary evaporator.
The solvent layer obtained was evaporated to dryness using a centrifugal evaporator (EYELA, Tokyo, Japan) set at 40 °C, and reconstituted in 5 mL of the mobile phase used for the HPLC MS/MS analyses.
The chloroform was evaporated using a rotary vacuum evaporator.
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