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Histological sections were graded independently by two observers unaware of animal treatment using an established scoring system for synovial hyperplasia (from 0: no hyperplasia, to 3: most severe hyperplasia) and inflammatory cells in synovial fluid (from 0: no inflammation, to 3: severe inflamed joint fluid).
Clinical scoring was performed using an established scoring system as described in Von Delwig's study [ 13].
The slices were examined by an experienced pathologist blinded to the treatment (AH) using an established scoring system [ 9, 30].
Acute Tubular Necrosis (ATN) was quantified using an established scoring system [ 16] by a blinded renal histopathologist.
Mice were assessed weekly for clinical manifestations of peripheral arthritis in a masked fashion using an established scoring method as previously described [ 4, 16].
The fiberscope score was classified using an established scoring system as follows: grade I = vocal cords not seen, II = vocal cords plus anterior epiglottis seen, III = vocal cords plus posterior epiglottis seen, and IV = only vocal cords seen [ 14].
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The severity of arthritis was assessed using an established semiquantitative scoring system, and the results are shown in Figure 1(c).
The severity of arthritis was assessed using an established semiquantitative scoring system of 0 4, where 0 = normal, 1 = swelling in 1 joint, 2 = swelling in >1 joint, 3 = swelling in the entire paw, and 4 = deformity and/or ankylosis [ 38].
CD3, CD68, Ki67 and TUNEL coded slides wereanalysed by two blinded operators using an established semiquantitative scoring method (0 4) (0=no staining, 1≤25%, 2=25% to 50%, 3=50% to 75%, 4≥75% staining).
There was significant impairment in the touch-evoked swim response in GFP CUG) 91 mRNA-injected embryos at 48 hpf compared with GFP mRNA-injected embryos using an established objective scoring system [ GFP mRNA, 2.86±0.05; GFP CUG) 91 mRNA, 2.06±0.11; n>50/group; P<0.001; Fig. 2B] (Dowling et al., 2009).
Motoric block was quantified using an established motor score derived from the Bromage score and adapted to rats [ 33].
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using an established cartilage
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com