Sentence examples for using an established quantitative from inspiring English sources

aureus GTB using an established quantitative PCR.

The presence of DNA double strand breaks was evaluated using an established quantitative indirect immunofluorescence-based approach (McManus and Hendzel 2005).

To verify the specificity of the constructs as well as to ensure that no compensatory effect involving increased expression of the others isoforms occurred, a secondary screen was performed at the mRNA level, using an established quantitative RT-PCR protocol (Czechowski et al. 2004).

Because this mutation did not impair caspase-8 cleaved recombinant BID (cBID) integration in purified mouse liver mitochondria (MLM) (Wei et al., 2000; Figure S1D), we could measure its biological activity using an established quantitative, specific cytochrome c release ELISA (Scorrano et al., 2002).

Histologic examination of tissue: Organs of athymic male mice stained with hematoxylin and eosin, trichrome, or giemsa were microscopically examined by a mouse pathologist (L.L.) in a blinded fashion, and toxicity scored using an established semi-quantitative histological scoring system38 (Supplementary Fig. 8).

Using an established, semi-quantitative scoring system, FITC staining of the corneal/conjunctival surface was evaluated by slit-lamp microscopic eye examination of each eye (n = 10), and the corneal and conjunctival areas were scored on a scale of 1 to 4 (shown in Additional File 6) by an observer unaware of the type of treatment given to each mouse.

This is essentially a generalized animal model, an established quantitative genetic method that has been used extensively to model biological and disease phenotypes.

Primers for quantitative reverse transcriptase PCR (qRT-PCR) were designed and tested for SYBR Green chemistry using an established in-house protocol [ 57].

Furthermore, instead of zymography, which identifies isoforms, we now used recently established quantitative bioactivity assays (BIAs) and specific antigen enzyme-linked immunosorbent assays (ELISAs) for MMP-2 and MMP-9.

To address this we used a set of established quantitative tests to assess behavioural and physiological phenotypes in mice (Hockly et al., 2003 a, b, 2006).

were tested for the presence of NV genogroup (GG) I and II infection using a sensitive and well established quantitative RT PCR method.