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In order to determine pS2 DNA fragment enrichment in MBD immunoprecipitated fractions, a dose-dependent and quantitative (Q-PCR) amplifications (Figure 2A, B) using an equal quantity of DNA (0.5 ng) per PCR assay, were performed with each fraction obtained from the ChIP procedure.
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MeJA (200 μM) was then added to the cultivation media; an equal quantity of ethanol was used as the control.
3 Mix PVA in a jar with an equal quantity of water - be careful not to use more glue than water, or the brown paper will never peel off your tiles.
Add the rosemary, wine and an equal quantity of water and simmer for 7 minutes.
Mix the crème de cassis with an equal quantity of the poaching syrup.
For the syrup, dissolve white caster sugar in an equal quantity of water.
If you don't have all these spices, replace them with an equal quantity of mixed spice or garam masala.
Security sources say an equal quantity of weaponry made its way to the IRA in four prior shipments.
Protein quantity was measured by Bradford assay and an equal quantity of total protein (15 μg) was applied on 10 or 12.5% SDS – PAGE.
Supernatants collected from all four extractions were mixed with an equal quantity of chloroform and isoamyl alcohol (25:25:1).
Reactions were incubated at room temp, then extracted with an equal quantity of EtOAc and analyzed by chiral-phase GC.
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