Sentence examples for using an equal dNTP from inspiring English sources

The (10× dNTP Mix) mixture was prepared by using an equal dNTP concentrations as follows: 2 µL of dATP (0.2 mM), 2 µL of dGTP (0.2 mM), 10 µL dCTP (1 mM), 10 µL dTTP (1 mM) and the distilled water was added until the volume of 100 µL.

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cDNA was regenerated through a random-primed reverse transcription using a dNTP mix containing dUTP.

Second strand synthesis was then performed using a dNTP mixture containing dUTP instead of dTTP.

Control DNA containing 5-hmC, mC, or C was amplified by PCR using a dNTP mixture containing dATP, dTTP, dGTP, and dCTP (Invitrogen), dm5CTP (New England BioLabs), or dhmCTP (Bioline).

Our results can be interpreted only using a scheme in which dNTP binding is required for kinetically productive DNA binding.

Self-rated proficiency was assessed using an analogue scale (0 100), where zero equals poor and 100 equals excellent.

Equal protein loading was confirmed using an α-tubulin antibody.

Radioactivity was measured in a Tri-Carb 2900TR liquid scintillation counter (Packard) and normalized to pmol/1×10 cells using a standard curve of known dNTP concentrations.

In the misincorporation assays with all four polymerases, an equal concentration of all the nucleotides (NTPs or dNTPs) was provided using a mix of four α-32P-NTPs or dNTPs (0.16 µM, PerkinElmer, specific activity, 3000 Ci/mmol) and nonradioactive NTPs or dNTPs (500 µM).

Fragments of 2 3 kb were isolated, end-repaired using a mix of natural and biotinylated dNTPs, blunt-end ligated using circularization ligase and fragmented to 200 400 bp.