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The motion of particles was imaged using an epifluorescent microscope (Nikon TE2000-S).
Cells were enumerated using an epifluorescent microscope and digital camera to determine the number of cells captured on the surfaces.
From this sample 10 µl was taken for direct counts using an epifluorescent microscope.
Slides were mounted with Vectashield and viewed using an epifluorescent microscope.
Sections were examined using an epifluorescent microscope with blue-violet excitation light set at 450 nm and 350 nm, respectively.
Observation and imaging were performed using an epifluorescent microscope (AxiophotZeiss Ltd., Germany) and a spinning disk confocal microscope (BX62 Olympus, Japan).
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After rinsing in PBGT, sections were mounted under coverslips in Vecta Shield (Vecta Laboratories Inc). and photographed using an Axiophot epifluorescent microscope (Zeiss).
Nuclei were counted in five random fields at 20 X magnification using an Olympus epifluorescent microscope.
The individual beads from the BEA reaction were then genotyped with two different fluorescent dyes specific for the wild-type or the ACH mutant allele and imaged as a monolayer bead-array using an automated epifluorescent microscope, as described previously (26, 28) (Fig. 1).
Images of metaphase spreads were captured using a Zeiss epifluorescent microscope with a six position filter wheel and were analysed using the Quips SpectraVision™ software (Abbott, as above).
Analysis was carried out using a Nikon epifluorescent microscope and a Q-imaging catera at ×40 magnification.
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