Sentence examples for using an enzymatic activity from inspiring English sources
Characterization of fatty acyl substrates for a potential acyltransferase using an enzymatic activity assay usually requires the purification of the protein or isolation of microsomal fractions as well as radioactive 14C-labeled glycerol 3-phosphate or 14C-labeled acyl-CoA [15] [18].
Levels of free MMP-2 and MMP-9 were determined in CSF using an enzymatic activity assay.
HRP quantities in the basolateral chamber, reflecting transcellular transit from the apical surface, was measured using an enzymatic activity assay with 3,3',5,5'-tetramethylbenzidine 3,3',5,5'-tetramethylbenzidinet de Claix, Freagent
The fifth group received Znpp alone before ischemia-reperfusion procedure, and the sixth group was administrated with a HO-1 inducer hemin prior to IR. HO-1 in the liver was measured using an enzymatic activity assay, a Western blot analysis, as well as immunohistochemical method.
Importantly, we found that apoptotic events induced by miR-29b were associated with reduced survivin levels and with caspase 3 activation, as revealed by increased caspase 3/7 activity using an enzymatic assay and by enhanced caspase 3 cleavage and reduced survivin expression in western blotting experiments.
G6P levels were determined using an enzymatic assay coupled to G6PDH activity [28], [29].
To verify that inducing expression of the lysozyme variants in the chemostat cultures was taking place and producing the expected consequences, lysozyme transcripts originating from the induction vectors were quantified using qRT-PCR and secreted lysozyme activity determined using an enzymatic assay (Fig. 2a, b).
PON-1 and ARE activities were determined using an enzymatic spectrophotometric method.
Recently, Guo et al. reported a novel approach to functionalize liposomes using the enzymatic activity of sortase A [ 79].
Refolding efficiencies were measured using the enzymatic activity assay at 5 nM initial RNase A concentration.
Consequently, an alternative approach is required to achieve reliable results when using microbial enzymatic activity for biomonitoring pollution status in aquatic sediments.
