Sentence examples for using an eluent mixture from inspiring English sources
The solvent was removed in vacuo, and the crude product was purified via silica gel flash-chromatography using an eluent mixture of ethyl acetate/hexane/TEA (500/500/0.8 (v/v/v)).
Purity of the 7A and 7B were assessed using an HPLC (Waters system 600 equipped with dual λ-detector 2487 set to 254 and 280 nm) with a C-18 reversed-phase column (Phenomenex Luna® C18; 100 Å; 5 μm; 250 × 10 mm) using an eluent mixture of acetonitrile and water as a gradient system (75% acetonitrile in water over 20 min) at a flow of 3 mL/min.
Both measurements used an eluent flow rate of 0.6 mL/min.
Finally, the residue was purified by Prep TLC using the eluent mixture (Hex/EtOAc = 60 40) to obtain the compound as a viscous yellow liquid.
One component of a polymer mixture is retained on the surface of an appropriate sorbent using an appropriate eluent while another component is chromatographed in the conventional s.e.c.e.c
The diethyl ether extract (65 mg) was separated by column chromatography on silica gel using a mixture of pentane ethyl acetate (4:1) as eluent to afford 1 mg of fucosterol (3).
Chromatographically pure MGDG was obtained from this crude polar lipidic fraction by flash chromatography in a silica gel column using a chloroform/methanol/water/acetic acid mixture (90 10 2.5 0.5 v/v) as eluent.
Besides, MIP-PVAm/CPVA microspheres have fine desorption property, and by using a mixture of ethanol and NaOH aqueous solution as an eluent, the desorption ratios can reach 99.73% as the effluent amount gets up to 20 bed volumes (BV).
After that, the separation of products was performed by silica gel preparative TLC using a mixture of CHCl3 : MeOH (93 : 7 v/v) as eluent.
The n-hexane fraction (3.76 g) was absorbed on a silica gel and chromatographed on a silica gel column using a mixture of hexane ethyl acetate of increasing polarity as eluent.
The product was purified by chromatography on a silica gel column using a mixture of hexane:ethyl acetate (10 1, v/v) as the eluent, giving a white solid (p.f.: 80 - 81 °C) with 76 % yield.
