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Dried, sieved, and weighed samples for each depth were analyzed for C content using an elemental combustion analyzer (Costech Analytical Technologies, Inc., Valencia, CA).
The aboveground shoot of each plant was ground and both %C and % N were determined for each plant using an Elemental Combustion System (Costech ECS 4010, Costech Analytical Technologies Inc).
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Total nitrogen was measured by dry combustion using an elemental analyzer (ISO13878), available P and K was determined after calcium acetate lactate extraction (CAL method; [30]).
Measurements were either performed on the pre-treated sample, using an elemental analyser, or on a CO2 aliquot from combustion or acidification.
Elemental analysis was performed using an Elemental Vario-III CHN analyzer.
Elemental analysis was performed by using an elemental analyzer (Vario EL Cube).
Contents of total nitrogen, carbon and sulphur in the dried powder of seed samples were measured were measured using an elemental analyzer (Vario EL; Elemental analyser system).
They used an elemental diet providing 900-1200 ml and calories daily.
Plant tissue %N was determined using a Eurovector Elemental Analyzer.
Carbon fraction was determined using a LECO-144 combustion chamber.
Total C and N were determined using a Europa elemental analyser (Sercon, Cheshire, UK).
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