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For each of the four maize samples, the extracted total proteins (15 μg) were separated on 12% SDS PAGE gels and then transferred onto a polyvinylidene difluoride (PVDF) membrane using an electrophoretic transfer system (Bio-Rad, USA).
Proteins were then transferred to a cellulose nitrate membrane using an electrophoretic transfer instrument (Bio-Rad).
For immunoblotting, proteins were transferred to polyvinylidene fluoride (PVDF) membrane using an electrophoretic transfer apparatus (Bio-Rad).
Total protein (40 μg per lane) was electrophoresed through a 10% SDS PAGE gel and transferred to a PVDF membrane (Millipore, UK) using an electrophoretic transfer chamber (Millipore, Walford, UK).
The extracts (50 μg/lane) were resolved by 3 7% NuPAGE Novex Tris-Acetate Mini Gels electrophoresis and electrotransferred onto an Immobilon polyvinylidene difluoride (PVDF) membrane (Immobilon-PSQ) using an electrophoretic transfer system (Invitrogen).
Resolved proteins were transferred to Hybond-P PVDF membranes or onto Immobilon-FL in 0.1 M Tris-base, pH 8.3, 0.192 M glycine and 10% (v/v) methanol using an electrophoretic transfer system with cold-block.
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The protein was transferred to PVDF immuno-blotting membranes using a Electrophoretic Transfer Cell (Mini Trans-Blot®, Bio-Rad) after being separated by electrophoresis in a Tetra Cell (Mini-PROTEIN®, Bio-Rad).
The extracted histone proteins were resolved on 15 % SDS-polyacrylamide gels, and subsequently transferred onto polyvinylidene fluoride fluoropolymer (PVDF) membrane using an electrophoretic blotting system (Bio-Rad).
Proteins were transferred to PVDF membrane (Millipore, India) from gel using an electrophoretic blotter (Bio-Rad).
The zeta potential of complexes was analyzed using an electrophoretic light scattering spectrophotometer ELS-80000, OTSUKA Electronics Co. Ltd., Japan) at room temperature to monitor the electrophoretic mobility of transfected complexes.
Pd nanostructure-decorated BiVO4 electrodes were prepared using an electrophoretic deposition process.
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