Sentence examples for using an electrophoretic mobility from inspiring English sources

To confirm this, we substituted Ser218 to aspartic acid to mimic phosphorylation (S218D) and assayed the ability of the mutant to bind DNA using an electrophoretic mobility shift assay.

The selected aptamers were characterized using an electrophoretic mobility shift assay, a quartz crystal microbalance, a fluorescence assay, and circular dichroism spectroscopy.

Activation of the nuclear transcription factor κB (NF-κB) was determined using an electrophoretic mobility shift assay (EMSA) [27, 28]: cell extracts were incubated with poly-doxy-inosinic-deoxy-cytidylic acid (poly-dI-dC) and 32P-labeled double stranded oligonucleotide containing the NF-κB (HIVκB-site) (5′-GGATCCTCAACAGAGGGGACTTTCCGAGGCCA-3′).

The DNA binding affinity for recombinant proteins from the wild-type, S98A and S98D plasmids were measured using an electrophoretic mobility shift assay (EMSA).

To further explore the potential RIG-I EGCG interaction we analyzed the RIG-I-agonist complexes using an electrophoretic mobility shift assay, followed by staining for the protein (Figure 6A).

NF-κB activation was assessed using an electrophoretic mobility shift assay (EMSA).

We used an electrophoretic mobility shift assay (EMSA) to examine whether OsSND2 bind to P9 and P3 fragments containing the SNBE1 and SNBE2, respectively.

We used an electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation analysis (ChIP) to further confirm that OsSND2 can directly bind to the promoter of OsMYB61 both in vitro and in vivo.

We used an electrophoretic mobility shift assay (EMSA) to test its binding to the cognate TIR (Fig. 3B).

To estimate the association rate constants (k1 in Figure 1I) we used an electrophoretic mobility shift assay (EMSA).

To determine how ZNF131 might affect the estrogen signaling pathway, we used an electrophoretic mobility shift assay (EMSA) to evaluate estrogen-dependent binding of ERα to the ERE of the Xenopus vitellogenin A2 gene in nuclear extracts of HeLa cells.