Spike sorting was performed offline using an automatic spike sorting software program with KlustaKwik (Harris et al., 2000) and FastICA (Hyvarinen, 1999) called 'ICSort', which is a custom-made program using Matlab and the C++ programming language.
Evaluation was performed using an automatic computerized system (Philips, NL Qlab software) and triggering was performed according to the Q wave of the ECG to enable measurement in complete relaxation of the ventricle.
The greatest distance between the lumen-intima interface and media –adventitia interface (IMT) is measured using an automatic edge detection and measurement computer software package, and mean and maximum IMT are recorded.
Morphometric analysis were performed on selected sections stained for distinct markers using an automatic image analyser employing Bioscan Optimas software (Optimas, Edmonds, CA, USA, Version 4.10) on a total of 50 glomeruli per animal; in a minority of samples with not enough glomeruli, cells were counted in at least 20 glomeruli.
In addition, we were able to obtain similar results by quantifying total GFP and mCherry fluorescence in full spinal cord tissue sections or individual cells using an automatic measurement of the intensity using ImageJ software from the National Institutes of Health (http://rsbweb.nih.gov/ij/), indicating that this method can be used to quantify LC3 flux with different image analysis approaches.
We used an automatic case-control matching software module to abstract 57 control patients from this group.
This study uses an automatic mesh generation algorithm with the software Simulation Ed. 2012 (Solid Works Corporation Concord, MA, USA), which provides a special element density at the plate-screw junctions three times that of the remainder of the model with an overall average element size of 2 mm.
After the release of the cuff the hyperemic response was recorded and the reactive hyperemia index (RHI) was calculated as a measure of endothelial function using the automatic software taking the relative difference between basic and hyperemic blood flow on the occluded arm and dividing it with the response on the control arm, to correct for any autonomic changes in vascular diameter.
To compare gene expression and differentiation, immunostained positive cells in the same region of the "peripheral" retina at the levels of the optic nerve plane were counted using Zeiss AxioVision automatic software.
DDoS attacks 'take down' websites by repeatedly loading them using automatic software.
