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The effective diameter of P. putida was determined using an automatic particle sizer (90Plus, Brookhaven Instruments Corporation).
Ribosome particles were windowed out from the micrographs using an automatic particle picking program (Rath and Frank, 2004).
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Cells were released from the surfaces using two sequential 10 m incubations in 0.25% trypsin at 37°C, to ensure that any remaining cells were removed from rough Ti surfaces, and counted using an automatic cell counter (Z1 Particle Counter, Beckman Coulter, Fullerton, CA).
Using an automatic coincidence correction guarantied that particles passing simultaneously were counted separately.
Thereafter, the sawdust of each species was classified into particle size (PS): PS ≤ 1 mm, 1 mm < PS ≤ 2 mm and 2 mm < PS ≤ 3.35 mm using an automatic sieve shaker with serial number A060-01/ZG/0038 and model A060-01.
Experimental pH was adjusted using an automatic CO2 injection system.
[ 17, 18] using an automatic analyzer.
Routine laboratory tests were conducted using an automatic biochemical analyzer and an automatic hematology analyzer.
Serum creatinine (SCr) was measured using an automatic biochemistry analyzer.
EMG and kinematics data were synchronized using an automatic trigger.
ABI was measured using an automatic oscillometric appliance.
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