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Samples were then processed using an automatic paraffin processor (Leica TP 1020, Leica Microsystems Inc., Concord, Ontario, Canada).
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Samples were fixed in 10% formalin, processed using an automatic tissue microprocessor, and paraffin-embedded.
For light microscopy, the samples were initially fixed in a 10% formalin buffer for 24 h at 4°C and were then immediately dehydrated in a graded series of ethanol, immersed in xylene and embedded in paraffin wax using an automatic processor.
Brains were then dehydrated overnight and paraffin embedded using an automatic tissue processor.
Conventional IHC staining on paraffin-embedded tonsillar tissue sections was carried out using an automatic processor and monoclonal anti-MLH1, anti-MSH2 and anti-MSH6 primary antibodies from Biocare Medical (Concord, CA); primary anti-AID antibody from Cell Signaling (Danvers, MA); and HRP-conjugated secondary antibody (Biocare).
Experimental pH was adjusted using an automatic CO2 injection system.
[ 17, 18] using an automatic analyzer.
Routine laboratory tests were conducted using an automatic biochemical analyzer and an automatic hematology analyzer.
EMG and kinematics data were synchronized using an automatic trigger.
ABI was measured using an automatic oscillometric appliance.
Trained personnel recorded blood pressure using an automatic device.
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