The amygdalae were segmented using an automatic, model-based segmentation tool (FSL FIRS T.
Total cholesterol, triglycerides, and HDL cholesterol were measured enzymatically using an automatic analyzer (model 736 40; Hitachi, Tokyo, Japan).
Blood lipid concentrations for total cholesterol, triglycerides, and HDL cholesterol were measured enzymatically using an automatic analyzer (model 736-40; HiTokyo, Japan, Japan).
Selected plasmids were isolated according to a standard alkaline lysis protocol, and single-pass sequencing of the 5'-termini was conducted with the primer TriplEx2-5' (CLONTECH Lab, Palo Alto, CA) using an automatic machine (Model 3100, Applied Biosystems, Foster city, CA) according to the manufacturer's instructions.
Aliquots of 50 µl of this solution were spread on Mueller Hinton agar plates in duplicate using an automatic spiral plater (model WASP 2, Don Whitley, Shipley, United Kingdom).
Amino acids analyses were made by the ninhydrin method using an automatic analyzer Beckman, model System 6300.
After homogenized in buffer (0.15 M NaCl, 5 mM EDTA, 0.1% Triton X-100, and 0.02 M Tris (pH 7.4)) using an automatic FastPrep homogenizer (model 120A-230, Thermo Savant, Holbrook, NY), the myofibrillar and collagenous proteins were precipitated by centrifugation (1600 g, 4°C, 20 min).
We measured BUN, serum creatinine, and uric acid using an automatic chemical analyzer (model TBA 40FR biochemical analyzer; Toshiba, Tokyo, Japan).
Together with a high level security policy defined by the security administrator, this augmented functional model is then used in an automatic model transformation to generate the platform specific security policy.
At least two positive colonies were sequenced by the dideoxy chain termination using an automatic DNA sequencer (ABI Applied Biosystems Model 3730).
PCR products were purified and sequenced in a forward and reverse direction by using an automatic nucleotide sequencer (ABI model 3730 sequencer, Applied Biosystems, Foster City, CA, USA).
