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After 30 min of agitation, the mixture was filtered through a nylon sieve of 200 μm and the spore concentration was evaluated using an automatic cell counter (Mishra et al. 2013).
At each time point, cells were trypsinized and counted using an automatic cell counter (Nexcelom, MA).
Cell counts were determined using an automatic cell counter (Nexcelom cellometerTM Auto T4, Bioscience LLC, Lawrence, MA).
Cells were sorted by flow cytometry using an automatic cell deposit unit (ACDU) in dry 96-well plates and directly frozen at -80°C overnight.
The haematological study (haemogram) was performed using an automatic cell counter (ABX 120DX Horiba, Spain).
Cells were counted on days 3, 5, and 8 by using an Automatic Cell Counter (Millipore, Billerica, MA).
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For quantifying the single-cell p53 traces, we used an automatic cell-tracking program that we developed using MatLab.
Erythrocyte count, hematocrit, hemoglobin concentration, and hematimetric indices (mean cell volume (MCV), mean cell hemoglobin (MCH), and mean cell hemoglobin concentration (MCHC)) were measured by using an automatic blood cell counter (Sysmex K1000; Sysmex, Hamburg, Germany).
Cells that had migrated to the lower surface were trypsinised and the cell number was determined using an automatic CASY cell counter (Scharfe system, Leipzig, Germany).
White blood cell and platelet count, hemoglobin and hematocrit were measured using an automatic electronic cell calculator.
Cells were trypsinised and counted after 12 h using an automatic CASY cell counter.
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