Measurements of IMT were performed off-line over a length of 10 mm beginning at 0-5 mm of the dilatation of the distal CCA using an automated edge detection reading system (Prowin software, Medical Technologies International, USA) by one certified reader.
IMT measurements were performed off-line over a length of 10 mm beginning at 0 5 mm of the dilatation of the distal CCA using an automated edge detection reading system (Prowin software, Medical Technologies International, USA).
A color-coded sequence unique to the reporter probe for each target RNA was read using an automated imager after elongation, alignment, and immobilization of the molecule using electrophoresis.
A single reading taken using an automated blood pressure monitor and using an appropriately sized blood pressure cuff will be recorded with the subject seated in a chair and after five minutes of rest.
Absorbance reading was performed using an automated reader (FLUOstar Galaxy, BMG Labtechnologies, Offenburg, Germany) and serum ECD concentrations were calculated from absorbance data using the Fluoscan Galaxy software (vers. 4.20-0, BMG LabtechnOffenburgOffenburGermanyany).
The optical density was read at 450 nm using an automated spectrophotometer (Spectra Mini; Tecan, Crailsheim, Germany).
After addition of acidic stopping solution (EUROIMMUN), the optical density (OD) was read at 450 nm using an automated spectrophotometer (Spectra Mini; Tecan, Crailsheim, Germany).
50 μl stop solution was added and the wells were read as Optical Density (OD) 450/620 nm using an automated reader (DTX 800, Beckman Coulter, USA) with cut-off limits calculated according to the instruction manual.
Using an automated gene finding and annotation pipeline (see construction and content), we identified 2,550 open reading frames (ORFs) in the B and 2,682 ORFs in the O genome.
The PCR product was sequenced using an automated DNA Sequencer (ABI3100 Applied Biosystems, Ipswich, MA, USA) with the forward primer to read 400 base pairs (bps) in one direction.
