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The absorbance was measured at 590 nm using an automated microplate reader (Biorad 680-XR, Japan).
The OTA content was inversely proportional to the colour intensity established using an automated microplate reader (EL × 800, BIOTEK, Instruments Inc., Winooski, VT, United States) at 450 nm.
The microplates were incubated in the dark at 28 °C for 10 days, during which period color development in each well was measured at the wavelengths of 595 and 750 nm, respectively, every 24 h using an automated microplate reader (BIOLOG, Hayward, USA) (Classen et al. 2003; Chen et al. 2013).
After 3 h (day0), 48 h (day2), 72 h (day4), 96 h (day6), the absorbance at the 490-nm wavelength was measured using an automated microplate reader (Bio-Tek Instruments).
After incubation at 37°C for 1 hr with constant agitation, wells were washed with 3×300 µl of PBST using an automated microplate washer, followed by the addition of 100 µl of PBS containing 1% (v/v) Tween 20 and 1 µg/ml biotinylated anti-PrP monoclonal antibody ICSM 35.
Absorbance at 450 nm was measured using an automated microplate reader.
Similar(39)
The absorbance of the plates was read at 450 nm using an Automated Microplated Reader (Bio-Tek, USA).
The colorimetric reaction was read using an automated ELISA microplate reader at 492 nm.
The formazan crystals formed were solubilized with SDS solution (100 μl of SDS-HCl solution (SDS 10% w/v, 0.01 M HCl)) and the absorbance (560-nm test/630-nm reference) was determined using an automated computer-linked microplate reader (Infinite M200 Pro microplate reader, Molecular Device Company, Sunnyvale, CA, USA).
The supernatant was transferred to a clean 96-well microplate, and the absorbance was measured at 570 nm using an automated 96-well microplate reader.
After this and each subsequent step, all wells were washed 3× with PBS with 0.05% Tween by using an automated EL 404 microplate washer (Biotek Instruments, Winooski, VT, USA).
More suggestions(15)
using an automated densimeter
using an automated sputter
using an automated compound-screening
using an automated ground
using an automated stepwise
using an automated flow
using an automated lickometer
using an automated biochemistry
using an automated computer
using an automated microwell
using an automated plate
using an automated peptide
using an automated labeling
using an automated freight
using an automated theorem
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