In November 2010, the Pennsylvania Bureau of Laboratories used an automated laboratory information system to send, by fax, a standardized questionnaire to 176 (86.6%) of the 203 clinical microbiology laboratories in Pennsylvania.
At admission blood glucose was measured in the laboratory using an automated glucose oxidase system (Analox instrument (Analox r) London, UK)).
In the present study, we demonstrate that the use of an automated laboratory-based assay for predicting GI overcomes the methodological and ethical constraints of in vivo testing and provides a practical solution to screening large volumes of samples.
Fibrinogen was measured by photo-optical clot detection based on the Clauss quantitative fibrinogen method, using an automated coagulation analyser (ACL Futura Plus, Instrumentation Laboratory, UK).
Biochemical serum parameters (creatinine, blood urea nitrogen, glucose, electrolytes, albumin and complete blood count calcium, phosphate, lipids, protein, cholesterol and triglycerides) were performed via the standard laboratory procedure, using an automated analyser.
The DCCT/EDIC Central Biochemistry Laboratory measured serum creatinine using an automated kinetic modification of the Jaffe reaction on a Beckman Synchron CX3 Clinical C System (12).
An automated laboratory incubation system was used to measure simultaneously N2O and N2, at 15 °C, with the moisture content raised by 3% (by weight) above the moisture content at field capacity (FC), giving a water-filled pore space (WFPS) of 80, 85 and 88% in the A, B and C horizons, respectively.
Platelet aggregation assays were performed in human platelet-rich plasma (PRP) and measured using an automated 4-channel Aggregometer (AggRAM version 1.1, Helena Laboratories, USA).
Target analytes and lipids were extracted using an automated modular solid-phase extraction system (Cambridge Isotope Laboratories, Andover, MA), which dried the sample onto the diatomaceous earth with pressurized nitrogen and eluted analytes and lipids with dichloromethane.
Serum iron, serum ferritin (Tina-quant Ferritin kit; Roche Diagnostics, Milan, Italy), hemoglobin, and glucose were determined using an automated COBAS C501 counter (Roche, Milan, Italy) at the clinical-chemical laboratory of the University Hospital of Modena.
