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Protein fractions were collected using an automated fraction collector.
One mL aliquots of radiolabeled virus were applied to the columns, and 10 drop fractions were collected using an automated fraction collector.
Peptide fractions were collected using an automated fraction collector.
Urine was collected and sampled every two hours throughout the experiment from the bladder catheter using an automated fraction collector.
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Microdialysis samples were collected in cooled plastic vials using an automated refrigerated fraction collector (CMA 470, Sweden).
The fractions were collected using an automated step-by-step fraction collector and guided for total carbohydrate using the phenol sulfuric acid method.
Soils were freeze-dried, lightly deconsolidated with a mortar and pestle, and sieved for 10 minutes to obtain the 63 μm fraction using an automated sieve shaker (Soil Test Engineering Model Cl-592B, SoilTest, Evanston, IL, USA).
Selected copolymers were also analyzed using an automated preparative molecular weight fractionation.
Lysate was clarified by centrifugation at 30.000 × g for 30 min and the protein contained in the soluble fraction was purified using an automated AKTA x-PRESS system on a nickel affinity column, followed by a desalting step using 3 × 5 ml HiTrap Desalting columns equilibrated in 50 mM HEPES pH 8.0, 1 mm tris 2-carboxyethyl phosphine (tris 2-carboxyethyl phosphine
Liver function tests (including total protein, albumin, total bilirubin, liver function enzymes (ALP, GGT, ALT and AST) and lipid profiles (including cholesterol fractions and triglycerides) will be measured using an automated Clinical Chemistry Analyser.
Radioactivity in fractions containing supernatant, cell-surface bound, and internalized radiopeptide was counted using an automated gammacounter system (Wizard, PerkinElmer, Waltham, MA, USA).
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