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All measurements were performed by one operator, blinded to all other data, using an automated computerized analyzing system [ 25].
The heart rate and the systolic blood pressure were measured using an automated computerized tail-cuff system in conscious mice following five consecutive training periods (Visitech BP2000, Visitech Systems, Inc., USA).
For locomotor behavior, adult male Wistar-Kyoto (WKY; n = 39) rats were given saline on experimental day 1 and either saline or an acute injection of MPD (0.6, 2.5, or 10.0 mg/kg, i.p). on experimental day 2. Locomotor activity was recorded for 2-h post injection on both days using an automated, computerized activity monitoring system.
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In week 14 and 22 systolic blood pressure was assessed by tail cuff method using an automated cuff inflator-pulse detection system (CODA2 Multi-Channel, Computerized, EMKA TECHNOLOGIES, Paris, France).
Systolic blood pressure as well as heart rate was assessed every two weeks by tail cuff method using an automated cuff inflator-pulse detection system (CODA2 Multi-Channel, Computerized, EMKA TECHNOLOGIES, Paris, France).
Six shoulders were tested using an automated mechanical system.
All but the ARG poll were conducted using an automated, recorded voice over the telephone.
Images were analyzed using an automated computer algorithm Quimp3.
Water and sucrose consumption were measured using an automated lickometer.
The purified sample was sequenced using an automated sequencer.
Liver enzyme activities were characterized using an automated biochemistry analyzer.
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