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The well-defined plateau during the oxidation step demonstrates high potentialities of this technique for electrochemical energy storage in nanoporous carbons using an aqueous medium.
Coverslips were then rinsed in distilled water, counterstained with DAPI for 20 minutes and allowed to dry before mounting using an aqueous medium.
Aortas were washed again in 60% propylene glycol and distilled water, mounted on glass slides, and coverslipped using an aqueous medium (Aquatex; Merck, Darmstadt, Germany).
Coverslips were then rinsed in distilled water, counterstained with DAPI for 20 min and allowed to dry before mounting, using an aqueous medium.
Sections were washed and incubated for one hour with a 1 200 dilution of a secondary goat anti-rabbit Cy3-IgG antibody (Jackson Immuno-Research Laboratories, West Grove, PA), then washed in 1 × TBS for 2 × 10 min. Slides were mounted using an aqueous medium containing Hoechst 33258 dye as a nuclear stain (Sigma, St . Louis MO).
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Samples were mounted using an aqueous mounting medium.
Finally, the slides were mounted using an aqueous mounting medium.
The slide was rapidly mounted using an aqueous mounting medium and digital images of microscopic fields acquired by Nikon Coolpix 950 digital camera (Nikon Instruments, Florence, Italy) after registering the X-Y coordinate of the acquired field in the slide.
The sections were counterstained with hemalun, mounted using an aqueous-based medium (Vectamount AQ, Vector), and analysed as described in Figure 3.
After counterstaining with hematoxylin and eosin, the slides were covered with glass coverslips using an aqueous-mounting medium (Crystal/Mount, Biomeda Corporation, Foster City, CA, USA), and representative images were obtained by contrast-phase microscopy.
Using an aqueous mobile phase of medium ionic strength, the elution was substantially regular and the macromolecules were not aggregate.
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