Based on these data an experiment in a continuous extraction column using an aqueous surfactant two-phase system containing d-glucose as additive was performed.
Polar-phase metabolites were extracted from homogenized fly tissue using an aqueous-optimized metabolite extraction protocol.
The earlier study used an aqueous tissue extraction [ 35], which would not prevent metabolic activity, especially as earthworm proteases are particularly active [ 52], and therefore their results may have included contributions from protein-bound histidine.
Firstly, the carob kibbles were submitted to an aqueous extraction to extract sugars, and supercritical fluid extraction (SFE) was applied to the solid residue of that aqueous extraction, by using compressed carbon dioxide (SC-CO2) as the solvent and a mixture of ethanol and water (80 20, v/v) as a co-solvent.
The oleosin fusion expression system is another example of fusion technology that has been used to produce recombinant proteins on the surface of oil bodies, which can be separated by flotation from an aqueous extraction of seed biomass [22].
The ground EBN then underwent an aqueous extraction process at a high temperature of 80°C before centrifugation and was then stored at 4°C until used.
Figure 2 shows the germination indexes (GI) after 48 h of incubation using different aqueous extraction ratios.
Ammonia nitrogen in tobacco was quantified spectroscopically using aqueous extraction followed by continuous flow analysis using a modification of the Bertholet reaction between ammonia, salicylate ions and dichloroisocyanurate, with nitroferricyanide as catalyst; the product, indophenol blue, was measured at 650 nm.
In work that was completed after this study had started, members in our group decided to use laboratory aqueous extraction conditions on a plant with excellent antifungal activities [ 54].
Tobacco reducing sugars were quantified using aqueous extraction of the STP sample, followed by continuous flow analysis.
