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Knockdown of CXCL-8 using an antisense vector resulted in increased cell death and reduced tumor growth relative to control.
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The present studies show that reduction of BRCA1 levels, using an antisense retroviral vector in the estrogen dependent BG-1 ovarian carcinoma cell line, may aid in confirmation of the hypothesis that BRCA1 functions as a tumor suppressor gene by playing a pivotal role in the balance between cell death and cell proliferation.
The present studies show that reduction in BRCA1 levels, using an antisense retroviral vector in the estrogen dependent BG-1 ovarian carcinoma cell line, contributes to confirmation of the hypothesis that BRCA1 plays a pivotal role in the balance between cell death and cell proliferation.
Nearly 50% reduction in PMCA2 or PMCA3 protein level was achieved using an antisense RNA cloned into pcDNA3.1 vector transfected to naive PC12 cells.
As a control, we used an antisense probe (Fig. 5F).
Nevertheless, it is unclear whether knockdown of RRIG1 expression using a vector carrying antisense open reading frame of RRIG1 can block expression of SH3GLB2 protein (Because no anti-SH3GLB2 antibody is available, we could not perform such an experiment).
Similar results were reported by Meiyalaghan [ 8] where the authors found no regenerant plants using antisense vectors expressing GSL1 (StSN1) and GSL2 (SN2) under their endogenous promoters regulation.
The mouse Rassf7 probes were generated using a pCMV.sport6 vector (IMAGE clone 4206694) and an SP6 (sense) or T7 polymerase (antisense) following digestion with StuI or AhdI enzymes respectively.
Luciferase activity was measured using a Vector 2 Luminometer (PerkinElmer Life Sciences).
Using an IPTG inducible mycobacterial vector, the vulnerability of this gene has been evaluated by antisense knockdown experiments in M. tuberculosis.
Both Luxturna and EDIT-101 use an AAV vector.
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