Sentence examples for using an antigen column from inspiring English sources

The peptide antibody was further affinity-purified using an antigen column.

Polyclonal anti-Eg5 antibody was purified from the serum of a rabbit (Eurogentec) injected with GST-Eg5 (constructed and purified as described [13]) using an antigen affinity column (Eg5 coupled to NHS-activated HiTrap (Amersham)).

Antisera were purified using an antigen affinity column prepared using concentrated BPK 282 spent cell culture.

The data were obtained using an antigen chip with 290 antigens (see Supporting Information S1).

Samples of each radioconjugate were routinely tested for immunoreactivity using a CEA antigen column.

Recombinant peptides corresponding to each C-terminus of the novel splice variants were used to immunize rabbits, and antibodies were affinity-purified from bulk sera over an antigen column.

RNA was then purified using an RNeasy spin column (Qiagen) and an on-column DNase treatment.

Pol IV was purified using a Capto S column, Hitrap Phenyl column, and Superdex 200 column.

Thin layer chromatography (TLC) was performed to assess iodine incorporation and antigen binding was assessed using a CEA column.

We extracted DNA using an ion-exchange column method [ 34].

2-MEA was then removed using a desalting column.