Sentence examples for using an analytical column from inspiring English sources

An 11 20% decrease in the average peak widths of nine peptides was obtained upon combining a preconcentration column with an analytical column as compared with a setup using an analytical column only.

To identify the furanocoumarin compounds in root extracts, 25 µl samples were separated by using an analytical column (Syncronis C18, 250 × 4.6 mm, 5 µm particle; ThermoFisher, USA) with flow rate 1.0 ml/min at 30 °C and absorbance 310 nm monitored by the ThermoFisher LC system (ThermoFisher, USA) equipped with a ThermoFinnigan UV detector and ThermoFisher Automatic Sampler.

We performed a subsequent analysis using an analytical column (Chromolith CapRod RP18e, 150 × 0.2 mm, Merck KGaA) by means of column switching to perform two dimensional orthogonal separations.

HPLC was performed using an analytical column (4.6 mm diameter × 25 cm long, particle size 5 mm) reverse phase C18 (Shimadzu, Japan) and a spectrophotometer UV/VIS SPD-20A detector (model Prominence, Shimadzu).

Some of the acid hydrolysates were also analyzed with a pulsed amperometric detector (871 Advanced BioScan, Metrohm AG, Herisau, Switzerland) to detect hemicellulose sugars as separate peaks, using an analytical column (Carbopac PA10; Dionex Co., Chelmsford, MA, USA) with an elution rate at 1.0 mL/min using a mixture of two eluents: NaOH 50 mmol/l and deionized water.

Peptide digests were then separated using an analytical column (Monolith, 0.1 mm×250 Monotechtech) at a 1 μl/min flow rate with a gradient in which the amount of solvent B was changed as follows: 5 8 min, 10%; 22 min, 40%; 23 min, 95%; 43 min, 95%; 44 min, 10%; and 59 min, 10%.

After lyophilizing combined eluates, 120 μL of water was added and 80 μL injected into high-performance liquid chromatography (HPLC), using an analytical amine column and acetonitrile/15 mM K-phosphate, pH 5.2 = 75/25 as the mobile phase.

Lyophilized fraction II (25 mg), containing PLA2 activity, was dissolved in 250  μL of 5% (v/v) acetonitrile in 0.1% (v/v) trifluoroacetic acid (TFA), homogenized and centrifuged at 480 ×g for 5 min, and then subjected to a reverse phase HPLC (model 2010, Shimadzu, Japan) using an analytical C18 column (Supelco, 250 mm × 4.6 mm).

Hydrolysates were analyzed for amino acids using an analytical ion exchange column (AA911, Transgenomics Inc., Omaha, NE, USA) and post column derivitization with ortho-phthaldialdehyde (OPA).

Further the purified CrSPI1-D1 was passed through the reverse phase chromatography using an analytical Jupiter C18 column.

The amount of aggregation of purified EF-Tu mutants was determined using an analytical Superose 6 column (GE Healthcare), and over 80% of the protein was monomeric.