Sentence examples for using an aliquot of from inspiring English sources
An emulsion for immunization was made by sonication, using an aliquot of human G6PI and complete Freund's adjuvant (Difco, Detroit, MI, USA), resulting in an emulsion of 2 mg/ml human G6PI.
Afterward, mercury vapor is generated using an aliquot of the slurry in the presence of the hydrochloric acid solution and isoamylic alcohol with sodium tetrahydroborate solution as the reducing agent.
Before incubation the initial activity was measured using an aliquot of 20 µl and adding 180 µl of reaction mixture (25 mM KPi pH 8.0 and 1 mM NAD+).
The erythro-magneto-HA-virosomes/R18 were solubilized by adding 0.1% (final concentration) Triton X-100 in PBS, and the fluorescence of R18 (excitation at 560 nm and emission at 590 nm) was measured using an aliquot of the solution with a spectrofluorometer Perkin Elmer 650-40), calibrated with R18 standard solutions containing 0.1% Triton X-100.
RT-PCR was performed using an aliquot of first-strand cDNA as a template under standard conditions with Taq DNA polymerase (QIAGEN).
If no product was obtained after the first amplification, a second PCR was done using an aliquot of the first reaction as a template.
We used an aliquot of the Canadian soil that had been adapted to SMZ biodegradation under field conditions [30], to enrich the microbial community, able to degrade SMZ in our lab.
Two types of purified ribosome preparations were used: we either used an aliquot of the ribosome containing fraction eluted from the QA column, or we concentrated the eluted ribosomes by centrifugation - 2 h at 31,000 rpm at 4°C, in a Beckman MLS-50 rotor (Beckman-Coulter) using an Optima Max tabletop ultracentrifuge (Beckman-Coulter) and resuspended them in ribosomal buffer prior to the assay.
Use an aliquot of cells and determine by immunofluorescence if the positive fraction is pure.
Use an aliquot of cells and determine by immunofluorescence if the negative fraction is devoid of fibroblasts.
As a reference, we also used an aliquot of the DNA of the original Kazusa strain, which had been stored as a stock in Kazusa DNA Research Institute.
