The deformability and aggregation of red blood cells were determined using a laser-assisted optical rotational cell analyzer (LORCA, RR Mechatronics, Holland) according to Hardeman's method [ 7, 8].
Increased CO2 concentration in the container was monitored using an infrared gas analyzer (Li-6262 CO2/H2O analyzer, LI-COR, Inc. USA).
Images were taken using an image analyzer (Typhoon, Amersham Biosciences).
The images were digitalized using an image analyzer (Chemimager, Biozym).
[ 17, 18] using an automatic analyzer.
Serum insulin was measured using an Immulite analyzer (Diagnostic Products).
Immunofluorescence was performed using an InCell Analyzer 1000 (GE).
Chemiluminescence was detected using an Image Analyzer LASS-3000- LASS-3000- Tokyo, Japan).
Routine laboratory tests were conducted using an automatic biochemical analyzer and an automatic hematology analyzer.
We measured BUN, serum creatinine, and uric acid using an automatic chemical analyzer (model TBA 40FR biochemical analyzer; Toshiba, Tokyo, Japan).
