Sentence examples for using an affinity tag from inspiring English sources

This differential thiol trapping with ICAT is followed by a tryptic digest of the proteins contained in the cell lysate and the purification of all ICAT-labeled peptides using an affinity tag.

We carry out these assays with recombinant POT1-TPP1 compurifiedrifrom from insect cells and bacteria respectively, and telomerase immuno-purified from HEK293T using an affinity tag and protease mediated elution after stringent washing steps.

For instance, epitope tags can be used as an affinity tag to perform protein purification when using stringent purification conditions or to identify interacting proteins when combining non-stringent affinity purifications and mass spectrometry analysis of co-purified proteins [ 16- 27].

Here, a modified histidine tag (HE) used as an affinity tag was employed together with a truncated maltotriose-binding protein (MBP; consisting of residues 59 433) from Pyrococcus furiosus as a solubility enhancing tag accompanying a tobacco etch virus protease-recognition site for protein expression and purification in Escherichia coli.

Because the C1A and C1B domains are notoriously unstable, maltose-binding protein (MBP) was routinely used as an affinity tag and was retained after purification, because of its ability to enhance the solubility of unstable proteins.

In order to determine whether this recombinant protein could be purified for further biochemical characterization, we proceeded further with the purification procedure using an affinity matrix adapted to the Strep-tag II (Figure 4B).

Purification of recombinant membrane receptors is commonly achieved by use of an affinity tag followed by an additional chromatography step if required.

To demonstrate the utility of the generated probes, we investigated their photo-crosslinking to proteins from nuclear extracts (see the Supporting Information) and successfully generated cross-linked ODN-to-protein conjugates that could be retrieved using the biotin modification as an affinity tag.

Furthermore, by fusing the Cap protein to an affinity tag, by using the appropriate preferred E. coli and by optimizing the codon usage of the polypeptide, it was possible to increase the yield of Cap fusion protein significantly.

To facilitate the detection of cross-linked peptides, one strategy is to selectively enrich cross-linked products for MS analysis using enrichable cross-linkers containing either an affinity tag (e.g., biotin tag) or a chemical handle that allows subsequent addition of an affinity tag through chemical conjugation.

These nonessential site(s) are then used as points of attachment to an affinity tag (e.g., biotin) or solid matrix (e.g., Affi-Gel agarose beads).