Sentence examples for using an affinity matrix from inspiring English sources

In order to determine whether this recombinant protein could be purified for further biochemical characterization, we proceeded further with the purification procedure using an affinity matrix adapted to the Strep-tag II (Figure 4B).

Using an affinity matrix, Seabra et al. [26] identified calprotectin as a target although this calcium binding protein is unlikely to be the primary receptor for 3-oxo-C12-HSL.

Purified, wild-type SEB (Toxin Technology, Sarasota, FL) was coupled to cyanogen bromide-activated Sepharose 4B (Sigma Chemical Co., St . Louis MO) and used as an affinity matrix.

Purified GST tagged proteins were bound to Glutathione Sepharose 4 Fast Flow resin and used as an affinity matrix for interacting protein capture from nuclear cell extract.

Indeed, our in vitro pull-down assay using GST EB1 as an affinity matrix confirmed the presence of an endogenous complex of TIP150 EB1 MCAK (Fig 4G).

The results obtained led to the identification of structure activity relationships further used to design an affinity matrix that can be used for fast and efficient purification of Eg5 from crude lysate of eukaryotic cells.

In Figure 1 you can see how the blurry black and white image gets clearer through the use of an affinity matrix.

In order to characterize the interaction between Wnt3a and Oto, full length Wnt3a was immunoprecipitated from transfected cell extracts using an HA affinity matrix.

Modules of transcripts associated with each trait with coordinated patterns of expression across the 40 lines were then quantified as described previously [ 83] by transforming the pairwise genetic correlations among transcripts into Euclidean-like distances, which were used to construct an affinity matrix.

Proteins expressed in HEK293 cells tagged with six histidines at the C terminus were purified using a Co2+ affinity matrix.

Immobilized Metal Ion Affinity Chromatography Proteins expressed in HEK293 cells tagged with six histidines at the C terminus were purified using a Co2+ affinity matrix.