Sentence examples for using an affinity column from inspiring English sources

Total RNA was isolated using a Trizol® extraction followed by purification using an affinity column (www.affymetrix.com).

The his-tagged protein was captured initially using an affinity column (Ni-NTA), which was followed by anionic exchange (Q resource) and finally gel filtration (Superdex 100) on a Pharmacia FPLC system.

By using an affinity column constructed by immobilizing E-selectin/Fc recombinant, the E-selectin binding protein that mediated breast cancer cell transendothelial migration was identified as human CD44 (Fig. 4).

A volume of 4 ml (2 g) frozen fly heads gave typically 45 mg of total MP with 0.5 mg HsSERT (1 mg DmSERT) purified routinely using an affinity column (Figure 3H).

Then DNA was purified using an affinity column, and subjected to PCR.

Using an affinity column with anti-SG antibodies to isolate adducts from proteolytic digests provides a concentrating step that greatly increases the sensitivity of detection.

While our lab used a single-stranded form of the inverted-repeat-containing elicitor response element as a bait [ 4], Vermel and colleagues used an affinity column exhibiting random bovine ssDNA sequences [ 25] and yet another group used an hexameric repeat of Arabidopsis telomere ends [ 26].

RNA was isolated using an affinity spin column (Qiagen) and 5 µg reverse transcribed into double stranded cDNA (Invitrogen) incorporating a T7 RNA polymerase promoter.

For purification and purity analysis of cMAb CK3, the pooled CK3 supernatant samples (∼1 L) were purified by the ÄKTAprime plus using an affinity chromatography column (HiTrap rProtein A-FF).

ERβ specific antibodies were purified from serum using an affinity purification column containing the ERβ protein fragment.

Completely assembled humanized antibody was successfully expressed in mammalian cells as IgG1 kappa molecules and purified using protein A affinity column.