Sentence examples for using an affinity capture from inspiring English sources

We have developed an unbiased method of quantifying reversible cysteine oxidation in proteins using an affinity capture technique we call Purification of Reversibly Oxidized Proteins (PROP).

To highlight genes of potential biological and clinical relevance in gastric cancer, we carried out a systematic array-based survey of gene expression and copy number levels in primary gastric tumors and gastric cancer cell lines and validated the results using an affinity capture based transcript analysis (TRAC assay) and real-time qRT-PCR.

Gilmore et al. [ 145] used an affinity capture technique in a microfluidic chamber for capturing and maintaining rotavirus double-layered particles (DLPs) in a liquid environment.

The product was then initially captured and purified from the clarified cell culture harvest using a protein A affinity capture chromatography step (MabSelect SuRe™; GE Healthcare Bio-Sciences ABjörkgatantan, Uppsala, Sweden) (Step 6), which binds to the Fc portion of the rFIXFc molecule with high affinity and specificity.

The his-tagged protein was captured initially using an affinity column (Ni-NTA), which was followed by anionic exchange (Q resource) and finally gel filtration (Superdex 100) on a Pharmacia FPLC system.

In addition, when used in an affinity capture-buffer exchange format the final samples are formulated in a buffer compatible with most assays without requirement of additional downstream processing.

The process employed multi-step purification and viral clearance processing, including use of a protein A affinity capture chromatography step, which binds to the Fc portion of the rFIXFc molecule with high affinity and specificity, and a 15 nm pore size virus removal nanofilter.

This involved an affinity capture of the cells using anti-CD66B antibodies, and stimulation with either bacterial lipopolysaccharide (LPS), or granulocyte-macrophage colony-stimulating factor (GM-CSF) plus interferon (IFNγ).

After protein A affinity capture, the Ig-containing eluate was further purified by membrane adsorption chromatography.

We then tested these predictions against the results of an affinity capture-MS experiment that used TAP-tagged Cbf11 as bait for the affinity purification.

The standard procedure for removal of linker DNA involves the prior labeling of linkers with biotin, which in turn enables them to be removed later using streptavidin affinity capture.