Vector pUC18 was cloned in One Shot TOP10 chemically competent E. coli (Invitrogen) and purified using an UltraClean 6 Minute Mini Plasmid Prep Kit (MO BIO).
The pDONR221 vector (Invitrogen Corp., CA, USA) was first propagated in One Shot ccdB Survival 2 T1R cells, a ccdB action resistant strain, and then isolated using an UltraClean 6 Minute Mini Plasmid Prep Kit (MO BIO Laboratories, Inc).
Finally, the viral DNA was further purified using an UltraClean DNA Purification Kit (MBI).
Synechocystis sp. PCC 6803 genomic DNA was obtained using an UltraClean Microbial DNA Isolation Kit (Mo Bio Laboratories, Solana Beach, CA).
DNA was extracted from respiratory isolates by using an UltraClean BloodSpin Kit (Mobio Laboratories, Inc., Carlsbad, CA, USA).
Harvested tissues were flash-frozen in liquid N2 and stored at -80°C, and RNA was extracted using an UltraClean plant RNA isolation kit (Mo Bio Laboratories, Cat. No. 13300-50) according to the manufacturer's guidelines.
PCR products were purified using an ultraclean PCR purification kit (DyeEx Kits, Qiagen, Hilden, Germany) and sequenced in an ABI 3130 × l sequencer using a Big Dye 3.1 terminator ready reaction kit (Applied Biosystems, California, USA).
Recombinant plasmids were purified using an UltraClean mini plasmid prep kit (MO BIO laboratories, California, USA) and sequenced using an automatic ABI PRISM 310 Genetic Analyser (PE Applied Biosystems, California, USA).
Recombinant plasmids were purified using an UltraClean mini plasmid prep kit (MO BIO laboratories, California, USA) and sequenced with an automatic ABI PRISM 310 Genetic Analyzer (PE Applied Biosystems, California, USA).
Most samples were prepared using an UltraClean Microbial DNA Isolation Kit (MO BIO Laboratories, Inc ., but DNA of strains HAMBI 2423 and HAMBI 2433 was prepared using the PrepMan Ultra Sample Preparation Reagent (Life Technologies), applying the protocol for preparation of samples for bacterial and fungal testing from culture broths.
