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RNA was isolated from crude homogenate according to the manufacturer's protocols (Trizol) and further enriched using an RNEasy column (Qiagen, Valencia, CA).
The resultant cRNA product was purified using an RNeasy column (Qiagen) and quantified with a spectrophotometer.
One-half of the cRNA product was purified using an RNeasy column (Qiagen) and quantified with a spectrophotometer.
Half the cRNA products were purified using an RNeasy column (Qiagen Inc., Valencia, CA) and quantified with a spectrophotometer.
Total cellular RNA was extracted using TRIzol (Invitrogen-Gibco) and purified using an RNeasy column (Qiagen, Valencia, CA).
One-half of the cRNA product was purified using an RNeasy column (Qiagen, Valencia, CA) and quantified with a spectrophotometer.
Similar(52)
RNA was resusupended in nuclease-free water and further processed using an RNeasy mini column with on-column DNase digestion (Qiagen, Valencia, CA) according to manufacturer's protocol.
RNA was then purified using an RNeasy spin column (Qiagen) and an on-column DNase treatment.
RNA was further purified using an RNeasy spin column (Qiagen, Chatsworth, CA) and an on-column DNase treatment.
Biotin-labeled cRNA was then purified using an RNeasy affinity column (Qiagen) and the cRNA was fragmented to ensure optimal hybridization to the oligonucleotide array.
Instead of using an RNeasy Midi column, we used phenol-chloroform extraction to collect a complete set of RNAs, including low-molecular-weight RNAs.
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